1. Run fast cool on the centrifuge
2. Add 0.1 volume 3M NaAc and 0.7 volume isopropanol to the eluted DNA
3. Mix well. Centrifuge at ≥15,000g for 30 min at 4°C
4. Discard supernatant, do not disturb DNA pellet
5. Wash DNA pellet with 70% EtOH
6. Centrifuge at ≥15,000g for 10 min at 4°C
7. Discard supernatant. Air dry 10 min
8. Add 65°C H2O/Tris to resuspend DNA

DNA Precipitation.pdf